Journal: Cellular and Molecular Neurobiology

Article Title: Neurotrophic Activity of Cultured Cell Line U87 is Up-Regulated by Proline-Rich Polypeptide Complex and Its Constituent Nonapeptide

doi: 10.1007/s10571-015-0192-8

Figure Lengend Snippet: Western blotting analysis of NGF forms secreted by astrocytoma cell line U87. Supernatants from control and compound-treated cells were separated in SDS-PAGE, then transferred to nitrocellulose membrane. NGF forms were detected with the use of specific polyclonal antibodies anti-βNGF. Immunocomplexes were visualized using a NBT/BCIP substrate and analyzed densitometrically in Molecular Imager ChemiDoc MP Imaging System with Image Lab Softwere (BioRad). The representative immunoblot is presented

Article Snippet: Rabbit anti-BDNF polyclonal antibody was from Bioss (MA, USA).

Techniques: Western Blot, SDS Page, Imaging

Journal: Neural Regeneration Research

Article Title: Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury ★

doi: 10.3969/j.issn.1673-5374.2012.10.004

Figure Lengend Snippet: Neurotrophic factor expression in surrounding injured brain tissues following human umbilical cord blood mesenchymal stem cell transplantation. (A–C) Nerve growth factor (NGF) protein expression (immunohistochemistry, × 200); (D–F) brain-derived neurotrophic factor (BDNF) protein expression (immunohistochemistry, × 200); (G–I) BDNF mRNA expression ( in situ hybridization, × 400). (A, D, G) Results (absorbance) are expressed as mean ± SD from six rats in each group at each time point. a P < 0.05, vs . model group ( t -test was used to specify differences between two groups at the corresponding time points); (B, E, H) model group at 14 days after transplantation; (C, F, I) transplantation group at 14 days after transplantation. Arrows: NGF protein-, BDNF protein-, and BDNF mRNA-positive cells.

Article Snippet: Primary antibodies were incubated overnight at 4°C in wet box with the following dilutions: rat anti-human BrdU monoclonal antibody (1:150; Beijing Boaosen Biotechnology, China), rabbit anti-mouse BDNF (1:150; Boster, Wuhan, China), NGF (1:150; Boster), VEGF (1:150; Boster), CD34 (1:100; Boster), bFGF (1:100; Beijing Boaosen Biotechnology) polyclonal antibody.

Techniques: Expressing, Transplantation Assay, Immunohistochemistry, Derivative Assay, In Situ Hybridization

Journal: Neuroscience letters

Article Title: NGF and BDNF expression in mouse DRG after spared nerve injury.

doi: 10.1016/j.neulet.2018.08.051

Figure Lengend Snippet: Fig. 2. Western blot analysis of NGF and BDNF expression in the L3-5 DRG. Relative NGF and

Article Snippet: After transfer, the membranes were blocked for 30 min with Blocking One-P (Nacalai Tesque), and incubated overnight at 4°C with rabbit antibodies raised against BDNF at 1:400 (ANT-010, Alomone, Jerusalem, Israel) and NGF at 1:1,000 (clone H-20, sc548, Santa Cruz Biotechnology, Santa Cruz, CA, USA) in phosphate-buffered saline containing 0.05% Tween-20 (PBS-T).

Techniques: Western Blot, Expressing

Journal: Neuroscience letters

Article Title: NGF and BDNF expression in mouse DRG after spared nerve injury.

doi: 10.1016/j.neulet.2018.08.051

Figure Lengend Snippet: Fig. 3. Immunohistochemical analysis of NGF and BDNF expression in the L3 DRG. (A) Typical

Article Snippet: After transfer, the membranes were blocked for 30 min with Blocking One-P (Nacalai Tesque), and incubated overnight at 4°C with rabbit antibodies raised against BDNF at 1:400 (ANT-010, Alomone, Jerusalem, Israel) and NGF at 1:1,000 (clone H-20, sc548, Santa Cruz Biotechnology, Santa Cruz, CA, USA) in phosphate-buffered saline containing 0.05% Tween-20 (PBS-T).

Techniques: Immunohistochemical staining, Expressing

Journal: Neuroscience letters

Article Title: NGF and BDNF expression in mouse DRG after spared nerve injury.

doi: 10.1016/j.neulet.2018.08.051

Figure Lengend Snippet: Fig. 4. Immunohistochemical analysis of NGF and BDNF expression in the L4 DRG. (A) Typical

Article Snippet: After transfer, the membranes were blocked for 30 min with Blocking One-P (Nacalai Tesque), and incubated overnight at 4°C with rabbit antibodies raised against BDNF at 1:400 (ANT-010, Alomone, Jerusalem, Israel) and NGF at 1:1,000 (clone H-20, sc548, Santa Cruz Biotechnology, Santa Cruz, CA, USA) in phosphate-buffered saline containing 0.05% Tween-20 (PBS-T).

Techniques: Immunohistochemical staining, Expressing

Journal: Neuroscience letters

Article Title: NGF and BDNF expression in mouse DRG after spared nerve injury.

doi: 10.1016/j.neulet.2018.08.051

Figure Lengend Snippet: Fig. 5. Immunohistochemical analysis of NGF and BDNF expression in the L5 DRG. (A) Typical

Article Snippet: After transfer, the membranes were blocked for 30 min with Blocking One-P (Nacalai Tesque), and incubated overnight at 4°C with rabbit antibodies raised against BDNF at 1:400 (ANT-010, Alomone, Jerusalem, Israel) and NGF at 1:1,000 (clone H-20, sc548, Santa Cruz Biotechnology, Santa Cruz, CA, USA) in phosphate-buffered saline containing 0.05% Tween-20 (PBS-T).

Techniques: Immunohistochemical staining, Expressing

Journal: Stroke

Article Title: Electrical Stimulation of the Cerebral Cortex Exerts Antiapoptotic, Angiogenic, and Anti-Inflammatory Effects in Ischemic Stroke Rats Through Phosphoinositide 3-Kinase/Akt Signaling Pathway

doi: 10.1161/strokeaha.109.563627

Figure Lengend Snippet: Figure 4. Upregulation of neurotrophic factors in the stroke rats receiving electric stimulation. The schematic diagram shows the regions in the cortex and striatum of rats with electric stimulation (A). Black box, cortex; white box, striatum. Western blots of BDNF, GDNF, and VEGF with -actin demonstrated the upregulation of all the factors both in the cortex and striatum of rats with electric stim- ulation compared with the control group (B). Quantification shown in C. Data represent mean percentagesSE. *P0.05 versus cortex of the control rats. **P0.05 versus striatum of the control rats.

Article Snippet: The membranes were probed at room temperature for 12 hours with mouse antivascular endothelial growth factor (VEGF) antibody (1:1000, #555036; BD Pharmingen), rabbit anti-glial cell line-derived neurotrophic factor (GDNF) antibody (1:500, #sc-328; Santa Cruz, CA), rabbit anti-brain-derived neurotrophic factor (BDNF) antibody (1:500, #546; Santa Cruz), rabbit antiphosphorylated Akt antibody (1:500, #9271; Cell Signaling), rabbit anti-Akt antibody (1:1000, #9272; Cell Signaling), and antimouse anti- -actin (1:2000, A5441; Sigma) as primary antibodies followed by exposure to peroxidaseconjugated secondary antibodies (1:2000, antimouse IgG; 1:2000, antirabbit IgG; Amersham Pharmacia Biotech; 1:2000) for 1 hour.

Techniques: Western Blot, Control